Midfielder Trotter missed Tuesday night's 2-1 defeat by Sheffield Wednesday with a knee injury and manager Kenny Jackett rates him 50-50 to feature at Wembley.
James Henry is in line to start after proving his fitness as a substitute against the Owls following a month out with knee ligament damage, which it was initially thought would end his season.
Fellow winger Martyn Woolford and on-loan Crystal Palace striker Jermaine Easter are cup-tied but defender Sean St Ledger, who recently signed on loan from Leicester, is available.
Danny Shittu leads the Lions out with club captain Paul Robinson still sidelined by a groin injury.
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