MacKenzie limped off with a knock after 20 minutes of the historic FA Cup fourth round victory over QPR on Saturday.
Tom Flanagan returned to the bench at Loftus Road after a broken foot and he is an option to replace MacKenzie at centre-half.
Izale McLeod will come back into manager Karl Robinson's squad after being cup-tied but fellow forward Daniel Powell (hamstring) remains sidelined along with long-term absentees Stephen Gleeson (broken foot) and Luke Chadwick (knee).
Midfielder Chris Lines will be hoping to make his league debut for the Dons after arriving on loan from Sheffield Wednesday last week.
- Sports & Recreation