"The Argentine forward... did physiotherapy work" apart from the rest of his team-mates, Barca said in a statement on their website (www.fcbarcelona.es).
Messi damaged a hamstring just over a month ago and although he had been given the all-clear by medical staff he was unable to feature in Wednesday's Champions League defeat by Bayern Munich.
Coach Tito Vilanova said he had not wanted to risk a more serious injury to the World Player of the Year and Messi had to watch from the bench as Barca were thumped 3-0 in the semi-final second leg for a humiliating 7-0 aggregate reverse.
Barca have an 11-point lead in La Liga over last season's champions Real Madrid in second and need five points from their remaining five games to secure a fourth title in five years.
They host Real Betis on Sunday and if Real Madrid fail to win at home to Real Valladolid on Saturday a Barca victory would be enough as they would be at least 13 points ahead with only 12 left to play for.
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