Top scorer Lisbie is still out with a hamstring injury and recent signing MacDonald is cup-tied having already played in the competition for MK Dons.
Millwall loanee Shaun Batt, who made his debut in Saturday's 1-0 win at Stevenage, is eligible to play and will partner David Mooney in attack.
Martin Rowlands and Lee Cook were rested at the weekend, although the latter came on as a substitute, and both should start against the Shrimpers.
Defender Nathan Clarke could also be involved after making his long-awaited return from an ankle injury as a late sub.
- Sports & Recreation
- Shaun Batt
- David Mooney
- Martin Rowlands