The Serbian, who will leave the programme after the Games, named seven foreign-born players in his squad, with goalkeeper Bobby White named as captain.
Jesper Parker and Robin Garnham are both Swedish-born, while German-born Chris Mohr is also included with Martin Hare and Steven Larsson, born in Norway.
French-born pair Seb Edgar and Gawain Vincent are also picked while former captain and playmaker Ciaran Williams, the only domestic-based player, makes it alongside line-player Chris McDermott, who had an injury interrupted season in Iceland.
Handball – an Olympic sport since 1972 – will be held at the Copper Box on the Olympic Park and Team Gb will throw-off against champions France on July 29.
Djukic – who also named Nicholas Satchwell as reserve – believes it was a difficult decision finalising his 14-man squad.
He said: “It was the hardest decision in my life, because our players have invested the last six years of their lives in the GB programme.
“We have a chance up to the quarter-final to change injured player with any player from the long list, (which) gave us the chance to reward the 15 players who invested the most over this period.
“In the end, the best 14 are selected and now it is time for concentration and to add the final touches for the start of the Olympic Tournament and the opening game against World and Olympic Champions France.”
Wirral-born McDermott believes selection is reward for the sacrifices he has made in his career since joining the GB programme.
“For me this selection means the world,” he said.
“I joined the handball program in 2006 and since then there have been so many ups and downs, but this makes all the nights sleeping on floors or couches so worthwhile
“To actually make it all the way and be selected for the Olympic Games was the best feeling in the world.
“However that is not to say the hard work is over, we are still in Serbia training at 100 per cent because we know the challenge that lies ahead of us won't be easy.
“Our official target is a top eight finish and if we can make that, great, but at this stage we need to be more focused the performance aspect of the games.”