Suzuki, meanwhile, will test with Randy de Puniet and Nobautsu Aoki as it continues preparations for a full-time return to the series in 2015.
Lorenzo, still recovering from the collarbone injuries he suffered at Assen and the Sachsenring, will partner team-mate Rossi for a two-day test at Brno.
Yamaha could also use the test to trial seamless transmission. Honda brought its own version of the system into operation earlier this year.
Across in Japan, Stoner's shock return to MotoGP action will be realised during a two-day test at Motegi.
The test has raised anticipation Stoner could make a wildcard return this year, either in Japan or his home Australia event, although Honda has categorically denied the possibility of Stoner competing.
Yamaha will also have a presence through test riders Katsuyuki Nakasuga and Wataru Yoshikawa, while Suzuki will join for both days.
"We are still in the testing and development stage of the bike," Suzuki team manager Davide Brivio told motogp.com.
"We will continue working with the data we collected at the previous tests of Motegi (in May), Barcelona and Aragon.
"The development has not stopped and this week we are going to try out various setups and continue to work on the electronics, chassis and engine packages in order to find further improvements in performance.
"We are still working with Mitsubishi on the bike, but analysis of the (compulsory) Magneti Marelli unit remains in progress.
"It is still not ready to be fitted to the prototype, but it will be in the near future."
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