Revenue increased by £42.9million from £110.6million to £153.5million during the 12-month period for 2012/13, driven by the full match programme and increased sponsorship.
The figures confirm the RFU's position as the world's richest union.
"This was an outstanding year in which continued growth allowed us to make record investment directly with clubs and in operating the English game at all levels," said chief executive Ian Ritchie
"We have made significant strides across the board allowing us to invest an additional £3.5million in grassroots rugby, largely thanks to a busy calendar of Twickenham events."
The RFU's annual report and accounts show investment in rugby as a whole rising to just under £64million, up 15 per cent on 2011/12.
Hospitality and catering is the single largest revenue stream and increased by £9.9million.
The retained profit for the year was £3.8million and the RFU's profit and loss reserve stands at £20million.
- Investment & Company Information