The South Korean came home in 37.63 seconds with Japan's Nao Kodaira finishing second to move third overall, while the USA's Heather Richardson came third to remain second, 70 points behind Lee's perfect score of 300.
However there was better news for Richardson 1000m as her run of 1:15.24 minutes was good enough to gone better, securing her second successive win.
Dutch skater Lotte van Beek's 1:16.15 minutes effort saw her finish third and move up to second overall 60 points behind Richardson, while Canada’s Christine Nesbitt came second, moving her up to fourth, just two points behind Dutch skater Marrit Leenstra.
Finland's Pekka Koskela heads the 500m men's World Cup after his run of 34.64 seconds handed him victory.
Michel Mulder of the Netherlands finishing second, while the USA's Tucker Fredericks came third.
Koskela is just five points ahead of Japan's Joji Kato in the overall standings, with Jan Smeekens of the Netherlands just a further five behind in third.
The Finn also tops the charts in the 1000m, his time of 1:09.52 minutes in Japan seeing him finish ahead of Germany's Samuel Schwarz and South Korea's Kyou-Hyuk Lee.
Koskela sits 35 points clear of Canada's Denny Morrison at the top of the standings, with Kjeld Nuis of the Netherlands in third place.
- Sports & Recreation
- Ice Skating
- Heather Richardson
- Pekka Koskela